The use of 125I as a membrane protein label for erythrocyte survival studies.

Radioactive iodide, incubated with erythlowing hemolysis of erythrocytes double rocytes in the presence of lactoperoxidase labeled with 1251 and 51Cr, radioactivity and hydrogen peroxide, is covalently from both these isotopes appeared mainly bound to membrane proteins. Using this in liver, lung, kidney, spleen, and urine. technique, rabbit erythrocytes were IaEnzymatic iodination provides a nonbeled with 1251 reinjected into the donors, eluting label of erythrocyte membrane and erythrocyte survivals determined. The proteins for the study of survival, sevalues for the erythrocyte half-lives and questration and turnover of this red cell the shape of the decay curves were corncomponent. parable to those reported using DF32P. Fol-